LC-MS?
   LIQUID CHROMATOGRAPHY                              MASS SPECTROMETRY
  •
   Separates                                         •
                                                       Component 
  components                                         identification is 
                                                     superior
  •
   Identification from 
  retention time is                                  •
                                                      But, interference 
  difficult                                          from other ions
  Ardrey, R. E. Liquid Chromatography Mass spectrometry: an introduction,          2
  Wiley, West Sussex, England, 2003
                                  HISTORY
      •  1906 : Mikhail Tsvet invented chromatography
      •  1930 : Edgar Lederer, Chromatographic
           separation of carotenoids
      •  1960s : Csaba Horvath, developed the first HPLC
      •  1990s: Engineering developments in HPLC
      •  2004:  UPLC and monolithic columns
     Henry, R. A. et al. In Liquid Chromatography In Clinical Analysis; Humana 
     Press: USA, 1981; p. 21-49                                                 3
                         Mass Spectrometry
      •   1897- Sir “J.J.” Thomson, charge/mass of electron 
      •   1919- Francis W. Aston with Thomson developed a “mass 
          spectrometer” to separate isotopes of elements
      •   1970s- Interfacing LC with MS
      •   1977-1980- Moving Belt Interface & Direct Liquid Interface
      •   1989- Electro Spray Ionization, John B. Fenn
                                                                   rd
      Watson, J. T. et al. In Introduction to mass spectrometry, 3  ed, Wiley:          4
      Wiltshire, 2007. 
                      COMPONENTS OF LC
           LIQUID                                                       SAMPLE 
        RESERVOIR                         PUMP
                                                                       INJECTOR
          RECORDER                      DETECTOR                        COLUMN
     Henry, R. A. et al. In Liquid Chromatography In Clinical Analysis; Humana       5
     Press: USA, 1981; p. 21-49
                                  COLUMNS 
   Height equivalent 
   to theoretical plate
        (HETP)                                  Height
     Backpressure overcome by,
    •Elevated temperatures
    •Monolithic columns
    Swartz, M.E. J. Liq. Chromatogr. R. T. 2005, 28, 1253-1263.                      6