Q:1:  The analytical technique in which 
              optics (Photometry) is NOT used in any 
              form:
              a. Atomic Absorption
              b.   HPLC
              c.   POCT for glucose
              d.   Radioimmunoassay
              e.   Real Time PCR
                                 Best answer: 
                           d. Radioimmunoassay
   Optical Techniques used in Various Instruments
   Atomic Absorption Photometry: Primarily a Photometric 
   Technique
   HPLC: Photometry used in detection in many types of HPLC
   Glucometers: Reflectance technique
   Real Time PCR:  Fluorometry in detection
   Q: 2. A Chemical Pathology autoanalyser has rejected the 
   blank cuvettes before the start of analysis with the 
   message “Cuvette Check Fail” (Dirty Cuvettes). Which of 
   the following phenomena is mainly prevented by this 
   function of autoanalyser:
   a. Absorbance of light
   b. Emission of light
   c. Reflectance of light
   d. Scattering of Light
   e. Transmission of light
            Best answer: 
          d.Scattering of Light
              Q:3:  Which of the following modifications makes 
              a Liquid Chromatography a High Performance 
              Liquid Chromatography (HPLC): 
              a.   Fixed loop injector 
              b.   Increasing volatility by sample derivatization
              c.   Length of columns from 50 to 250 nm
              d.   Small-diameter particles as stationary phase
              e.   UV lamp detectors of 190 to 400 nm. 
                                  Best answer: 
          d. Small-diameter particles as stationary phase
              Q:4:  It is reported that the sensitivity of an Atomic Absorption 
              method is 100 times higher than Flame Emission. The reason for 
              this very high sensitivity is:
              a.   Drastic reduction in background noise
              b.   Most of the ground state atoms absorb light.
              c.   Sample derivatization before analysis
              d.   Use of a hollow cathode lamp made of the material to be 
              analysed.
              e.   Very high temperature is used for excitation of atoms.
               
                                  Best answer: 
       b. Most of the ground state atoms absorb light.