Overview
      Southern-blot               DNA Digestion Based
      hybridization
                                       COBRA
                                                                    MSP
        Bisulfite Sequencing
                                   Bisulfite Based              Real-time MSP
                                                                 MethyLight
            MassARRAY
                                    Pyrosequencing
                                    Southern Blot Hybridization
                                                    1978
         -One of the older techniques for sequencing (1978), not as widely used any more due to the
         development of PCR and other cheap/fast sequencing techniques,  not outdated but just not as
         widely employed 
         Process:
             •    Step 1 use restriction enzymes to cut DNA
             •    Step 2 electrophoresis to separate DNA fragments by size
             •    Step 3 transfer DNA fragments from gel to a membrane
             •    Step 4 hybridize radiolabeled probe to membrane
             •    Step 5 sample is washed to remove extraneous radio probes 
                                             Southern Blot Hybridization
                                                                 1978
           Advantages:
               •    Cheap
               •    simple
               •    1 probe can detect multiple similar sequences 
               •    well suited for analyzing long stretches of DNA 
           Disadvantages
                •   Requires a large amount of DNA
                •   Time consuming 
                •   labor intensive 
                •   limited sensitivity 
           Interesting probe gif 
           http://www.slic2.wsu.edu:82/hurlbert/micro101/images/101SouthernBlot10.gif