Announcements
   • Set 1 Lab Reports 
    – hand back
   • Set 2 Lab Reports
    – Due 4/10
   • Final Exam – April 15th
   • Future Mondays (after 4/15)
    – Set 3 Presentation – 4/29
    – 4/22 and other Mondays 
      • can’t start lab at 5:00 in Sequoia 538 (Chem 125 
       conflict)
      • can run lab from 5:30 to 9:00 or
      • 5:30 to 8:30 plus Wed from 5:00 to 8:00 or
      • first 30 min of lab in Sequoia 540
       Labs – More Details
  • Set 2 Lab Reports
    – Real sample analysis will count for at most 
     25% of points (less for HPLC lab)
  • Set 3 Labs
    – We have tristearin and tripalmitin that can be 
     used as recovery standards
    – We also have old linoleic (C18:2) and linolenic 
     (C18:3) acid standards that can be used as 
     qualitative standards (these standards 
     decompose easily)
    – These are in 540 freezer
    – You should be deciding on your “real” sample 
     soon and let me know what other equipment or 
     standards you will need
       Labs – More Details
  • Set 3 Labs – cont.
    – Tentative presentation and paper due dates
    – Presentation 4/29 (1 week after finishing lab)
    – Paper due 5/6
    – Presentation should be focused on your sample 
     of interest and should include some literature 
     research (why is this sample of interest and 
     what analysis has been done previously)
        Labs – More Details
   • Term Project
    – You should figure out what you plan to do 
      before next Monday
    – Two options:
      • isolation of significant ingredient from household 
       product (best for 5 to 50% that ingredient)
      • use of “new” equipment for analysis of compound 
       from sample (e.g. use of HPLC-fluorescence detection 
       for capsaicin in chile samples or analysis of caffeine 
       in a beverage by SPME-GC-NPD)
    – I will have a sign up
      • limit to number of students using particular 
       equipment for bulk of work (not a problem for 
       isolation projects)
      • only 1 student per household sample/ingredient
      • you may want to have a back-up compound/plan
       Final Exam - Review
  • Example posted on-line
  • 15% of total grade
  • Can use lab notebook and notes from lab 
    lecture (including slide)
  • What to know – from 1/28 lecture
    – Understand goals of method optimization and 
     measures of how well that is accomplished
    – Basic safety rules
    – How to transfer data (raw, chromatograms, 
     and software table data) to Excel
    – Steps to turning on and off HPLCs and GCs