PRE-LAB
       Sign-in 
       Quiz (Benoit. 2005)
                                                  BME 245/445 – Lab 3
      LINEAR MOLECULES
       PEGMA
       Acr-PEG-RGDSG
                                        Modified from (Lin, C.C., Pharmaceutical Research. 26(3): 
                                        631-643. 2009)
                                                       BME 245/445 – Lab 3
       SECTION 1- Done before lab
       PEGMA Network Synthesis (done before lab – as previous)
       1.  10wt% PEGMA in PBS
          1.  +/- 5 mM Acr-PEG-RGDSG
       2.  10% photo-initiator by volume (LAP) 
       3.  0.75 mL Placed between two glass slides with spacer
       4.  Exposed to UV-lamp for 10min
       5.  Remove, punch out cylindrical samples
       6.  Place in PBS
                                                            BME 245/445 – Lab 3
       SECTION 2
    Cell Culture (TA demo and then hands on)
     Sterile Techniques
     Feeding cells
     Counting cells
     Seeding cells (TC plastic / PEG / PEG+RGDSG)
     Monitor cell-material interactions over time… (ImageJ)
         # of adherent cells
         Average adhesion area
         Average spindle factor
                                                           BME 245/445 – Lab 3
       SECTION 3
       Time-course data collection
        Days 0, 1, 2, 3
            # adherent cells, adhesion area, spindle factor (ImageJ)
        Day 2
            Live/Dead imaging
                                                                BME 245/445 – Lab 3