Leishman Stain                                                                                                                                                                         
                              
                             Intended use 
                             Leishman's Stain is used generally in hematology and tropical medicine to differentiate and identify leucocytes, 
                             malarial parasites and trypanosomes. 
                              
                             Summary 
                             Leishman stain is used in microscopy for staining blood smears. It provides excellent stain quality. It is generally 
                             used to differentiate and identify leucocytes, malaria parasites, and trypanosoma. It is based on a methanolic 
                             mixture of "polychromed" methylene blue. (i.e. demethylated into various azures) and eosin. The methanolic stock 
                             solution is stable and also serves the purpose of directly fixing the smear eliminating a prefixing step. 
                              
                             Principle 
                             Leishman stain contain methylene blue dye, a basic dye, which gives colour to an acidic component and eosin 
                             dye, an acidic dye, which gives colour to a basic component. These dyes differentiate the different component of 
                             blood.  Leishman's Stain belongs to the group of Romanowsky stains. For the detection of malaria parasite 
                             Leishman staining seems more sensitive than e.g. Field's stain. 
                              
                             Reagents / Contents  
                             Leishman stain                              0.15 g  
                             Methanol, absolute                          100 mL  
                              
                             Appearance: Clear blue colour solution. 
                              
                             Storage and stability  
                             Store at 15°C - 25°C away from bright light. Use before expiry date on label. 
                              
                             Materials required but not provided 
                             Clean grease-free glass slide, loops, staining rack, blotting paper, immersion oil, and microscope. 
                              
                             Type of Specimen 
                             Clinical specimen: Peripheral Blood samples. 
                              
                             Procedure 
                             1. Use blood smears that are as thin as possible and air-dried.  
                             2. Fully cover the smear with 1:1 solution of Leishman’s Stain in buffered water for 8-10 minutes.  
                             3. Rinse gently with distilled water for 1 to 2 minutes till no more stains flows.  
                             5. Dry the slides using blotting paper and air-dry. 
                             6. Observe under microscope, 40X and 100X under oil immersion lens. 
                              
                             Interpretation of results  
                             Erythrocytes:                                             Light pink to brown 
                             Cores of lymphocytes:                                     Deep, dark blue to blue-violet 
                             Cytoplasm of lymphocytes:                                 Light blue 
                             Nuclei of neutrophil:                                     Deep blue to blue-violet 
                             polymorphonuclear leukocytes 
                             Granules of neutrophilic:                                 Red 
                             polymorphonuclear leukocytes 
                             Cores of eosinophil leukocytes:                           Blue violet 
                             Granules of eosinophilic leukocytes: Deep red 
                             Cores of basophilic leukocytes:                           Blue violet 
                              
                              
                Warranty 
                This product is designed to perform as described on the label and pack insert. The manufacturer disclaims any 
                implied warranty of use and sale for any other purpose. 
                 
                Reference 
                                     ®
                Data on file: Microxpress , A Division of Tulip Diagnostics (P) Ltd. 
                 
                Product Presentation: 
                Cat No.                        Product                                Pack Size 
                207120670250                   Leishman Staining Kit                  2 x 250 mL 
                207120670500                                                          2 x 500 mL 
                207120420250                   Leishman Stain                         250 mL 
                207120420500                                                          500 mL 
                207120421000                                                          1000 mL